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After an additional twenty-four hours, cells were detached by trypsinization, washed in PBS and frozen until lipids were extracted

The Bowers and colleagues canonical work across the 1980s and 1990s reported that co-administration of a GHRH-R agonist with a GHRP-class agent produced greater growth hormone release than either compound alone in healthy human volunteers, with the synergy mechanism interpreted as pulse-amplitude (GHRH-R) versus basal-frequency (GHS-R1a) control of pituitary somatotroph release plus differential engagement of the somatostatin tone [5]

Slices were then transferred to standard artificial cerebrospinal fluid (aCSF

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