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Exposing the brain of either vehicle- or DMF-treated animals to CDNB in these conditions did not induce any detectable alteration in the staining of either claudin-1 or claudin-2, two major proteins associated in the brain with choroidal epithelium tight junctions, as assessed by epifluorescence and confocal microscopy (Additional file 2)

2.4 Morphological analysis A375 human cells (1.5 10 5 ) were seeded in 60 mm plates and grown in standard conditions (see paragraph cell culture) for 24 h at 37 C with 5% CO 2