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The role of NAD+ in cellular energy and repair mechanisms highlights its importance

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As an example illustrating the present invention, histidine has a predominant positive net charge below pH~6.5, thus for cation exchange the pH elution gradient with a solvent comprising an organic solvent or modifier could begin below pH 6.5 to remove a truncated form missing histidine and end the gradient above pH 6.5 thereby subsequently eluting the target GLP-1 moiety