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The concentrate was precipitated in cold diethyl ether (40 mL), centrifuged, and the pellet dissolved in 5 mL H2O:ACN (1:1, 0.1% TFA)

[3] [5] If you are participating in a clinical trial involving a GLP-1 patch, you must follow the specific instructions provided by the research team

Briefly, the initial small increase of SHs stimulates GH secretion in the early phase of puberty, whereas during late puberty, the further increase of SHs determines bone ossification and, therefore, the arrest of growth [18]