For GSH assay, cells were treated in Earles balanced salt solution (EBSS), supplemented with 25 mM glucose, 4 mM glutamine, 1 X MEM vitamin solution (Life technologies, CA, USA), 0.22% wt/vol NaHCO 3 , 1 mM sodium pyruvate, 0.2% BSA, 25 mM HEPES, 100 U/ml penicillin and 100 g/ml streptomycin in the presence or absence of 1 X MEM amino acid solution (Gibco, CA, USA) with or without 100 M cystine
The number of subjects chosen was guided by our previous demonstration of bioavailability of SF from GR alone and GR + Myr broccoli seed and sprout preparations 18,35,59
At this concentration, luteolin significantly suppressed phosphorylation of c-Jun at Ser63 and Ser73 residues in nuclear extracts, with no effects on p-Syk, p-Lyn, or p38 MAPK, p44/p42 MAPK, or p54/56 SAPK/JNK in cytoplasmic, or c-Jun in nuclear fractions[170]
No significant correlation was found between metabolite levels and hyper/impulsive scores of ADHD, measures of core ASD symptoms (ADOS-2, SRS-P) or adaptive behavior (ABAS-2)