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30 l of the lysis buffer (1 mM TrisHCL, pH 7.4, 1 mM EDTA, and 0.5% Triton X-100) was added to each well using a multichannel pipette and mixed gently, with 5 l from each well mixed with 195 l of 1:5 Bradford reagent (Bio-Rad) for protein quantification

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