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In-gel digest and MS Coomassie-stained gel pieces were digested in gel with trypsin 57 , and desalted 58 peptide mixtures were analyzed on an Easy-nLC 1200 coupled to a Q Exactive HF mass spectrometer (both Thermo Fisher Scientific) as described previously 26 with slight modifications: peptide mixtures were separated using a 57-min and 87-min segmented gradient, respectively, of 10%33%50%90% of HPLC solvent B (80% acetonitrile in 0.1% formic acid) in HPLC solvent A (0.1% formic acid) at a flow rate of 200 nl min 1

New England Journal of Medicine, 384(11), 989-1002

There isnt any published evidence supporting that it is a gut peptide.

Levels of this peptide decline with age, which is one reason the bodys ability to repair tissue, produce collagen, and recover from inflammation slows over time