Splenocytes from -MYC mice and wild-type littermates were isolated as previously described [53] and stained with APC-conjugated rat anti-mouse CD45R/B220 (ref 561880) and PE-conjugated rat anti-mouse CD43 (ref 561857) antibodies, both from BD Biosciences, before flow cytometry analysis with a FACS Canto II flow cytometer (BD Biosciences) employing Kaluza software (Beckman Coulter)
J Biol Chem 277:3212432132 Deng C, Sun Z, Tong G, Yi W, Ma L, Zhao B, Cheng L, Zhang J, Cao F, Yi D (2013) -Lipoic acid reduces infarct size and preserves cardiac function in rat myocardial ischemia/reperfusion injury through activation of PI3K/Akt/Nrf2 pathway
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Those receiving 5 mg, 10mg, and 15 mg experienced an average reduction of 7.6 kg (about 17 pounds), 9.3 kg (about 20 pounds), and 11.2 kg (about 25 pounds) respectively