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Microcomputed tomography (CT) and histomorphometric analysis Femura and tibiae obtained from mice were dissected free of soft tissue, fixed overnight in 10% neutral buffered formalin for 24 h, dehydrated in 70% ethanol for 3 days, and analyzed by a high resolution CT (SkyScan 1076 in-vivo CT, SKYSCAN) installed with softwares NRecon v1.6, CTan v1.9, and CTVol v2.0

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The feeding medium was continuously added into the bioreactor to obtain a constant specific growth rate (0.25 0.02 h 1 ) after the initial glucose in the medium was completely consumed

Folate (vitamin B9) is very important to brain development