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Quantitative PCR was performed in technical triplicate using 20 ng cDNA, 1 Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) and 0.5 M forward and reverse primers (Supplementary Table 1), using an ABI 7900HT Real-Time PCR System (Applied Biosystems)

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The Mechanism: Phentermine is a top-down approach, using brain chemistry to blunt hunger signals