Due to the pure vitamin C content, the packaging warns that there may be discoloration and changes in odor at high temperatures or over time, though this doesn't affect the product's quality
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Materials and methods Cell culture and compound treatment HepG2 cells (ATCC, catalog: HB-8065) were cultured with 90% Eagles Minimum Essential Medium (EMEM, ATCC, catalog: 30-2003) and supplemented with 10% fetal bovine serum (FBS, Cytiva, catalog: SH30070.03) and 100 U/mL penicillin-100 g/mL streptomycin (Thermo Fisher Scientific, catalog: 15140122)
The intestinal barrier consists of four components: microbial barrier, biochemical barrier, physical barrier and immune barrier [8]