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The compound's primary target, NNMT, sits at a critical metabolic intersection where NAD+ precursor availability meets cellular methylation capacity

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In diet-induced obese mice (16 weeks HFD), subcutaneous 5-Amino-1MQ treatment for 11 days (20 mg/kg/injection, three injections daily) produced significantly reduced body weights, white adipose tissue masses, and adipocyte cell sizes compared to vehicle controls