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Cells were incubated with primary antibodies (rabbit anti-rat IgG) overnight at 4C

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Tissues were blocked in CAS block (Thermo Fisher Scientific) and incubated overnight with primary antibodies diluted in CAS block (Table 1): Primary antibodies for immunofluorescence Slides were washed 3 times in PBST, incubated overnight with secondary antibodies, and diluted in PBS/BSA 1% (Table 2): Secondary antibodies for immunofluorescence Slides were then washed once in PBS and closed with cover glass using ImmuGio mounting medium (DiaSorin, 2505-5)