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301 R1250R1258 10.1152/ajpregu.00841.2010 [DOI] [PMC free article] [PubMed] [Google Scholar] Miller W

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Tissues were homogenized in cold RIPA lysis buffer (Beyotime, Shanghai, China) containing phenylmethylsulfonyl fluoride (PMSF, Beyotime, Shanghai, China) at 4 C for 1 h