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2.4.2 Analysis of blood antioxidant indexes Preparation of serum: 7 bullfrogs were taken from each of the NC group, FI group and GSH group for blood sampling, placed at room temperature for 4~5 h

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Apart from the GSH-recycling method, GSSG is generally reduced to GSH by thiol exchange type reagents (like dithiothreitol) or by phosphines and then the thiol group is labeled as mentioned above

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