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Gradual titration is essential: Starting low (around 0.25 mg weekly) and stepping up slowly markedly lowers the odds of early gastrointestinal effects [4]

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Further analysis of adipose tissue showed that adipogenesis-related genes ( Acaca , Fsn , Fabp4 , and Srebf1 ) were downregulated, while lipolysis-related genes ( Lipe and Adipoq ) and cholesterol homeostasis regulatory genes ( Ldlr and Abca1 ) were upregulated by nicotine treatment (Supplementary Figures 2D)

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