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Medications Many common and regularly used medications will affect the absorption of Vitamin B12, this includes the following drugs: Acid lowering drugs known as PPI used to treat reflux Antibiotics Metformin, which is a common drug used to treat diabetes Anti-seizure medications Antidepressants affect folate levels and this can have an influence on B12 levels Note: If you are taking these medications and have low B12 do not stop your medication without consulting your doctor

The immobilization enzymes were washed three times with 2 mL of 20 mM potassium phosphate buffer (pH 8.0)

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