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Each vitamin plays a unique and irreplaceable role in the body: Vitamin B6 : This vitamin is essential for protein metabolism, the production of neurotransmitters such as serotonin and dopamine, and the synthesis of hemoglobin , which is crucial for oxygen transport in the blood

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That rules out, straightforwardly, radioligand or fluorescent binding assays, competition binding against a known ligand, recombinant receptor-expressing lines, functional readouts tied to a defined coupling pathway, and antagonist controls to demonstrate that an effect is receptor-mediated