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The transmembrane region of GLP-1R was predicted using PPM 3.0 Web Server (71) and embedded into an asymmetric lipid membrane bilayer in a 181817 nm 3 box using insane.py (72), with lipid composition as follows: POPC (30%), DOPC (30%), POPE (8%), DOPE (7%) and cholesterol (25%) in the outer leaflet and POPC (5%), DOPC (5%), POPE (20%), DOPE (20%), POPS (8%), DOPS (7%), PIP 2 (10%) and cholesterol (10%) in the inner leaflet