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SKOV3 cells were plated and grown to near confluency, and T cells isolated from a healthy donor (M46, 46-year-old male) were added at an effector-to-target (E:T) ratio of 10:1

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Moreover, the first global genome-wide epigenetic analysis in VAT 211 from IR and insulin-sensitive (IS) morbidly obese patients identified a novel IR-related gene, the zinc finger protein 714 (ZNF714) exhibited the highest DNA methylation difference, and its methylation levels is lower in IR patient than in IS patient, consistent with increased transcription levels, such studies provide potential epigenetic biomarkers related to IR in addition to novel treatment targets for the prevention and treatment of metabolic disorders

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