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Preserves post-translational modifications (PTMs) Produces c-ions and z-ions Ideal for analyzing modified peptides and large biomolecules When to Use Which Technique Practical Insight Use CID or HCD for standard GLP-1 peptide sequencing workflows Use ETD when analyzing peptides with labile or complex modifications Combining multiple fragmentation techniques can significantly improve sequence coverage and confidence 7: Importance of Detecting Modifications in GLP-1 Peptides Detecting modifications is crucial in Peptide Sequencing of GLP-1 Peptide because structural changes directly influence drug efficacy, stability, and regulatory compliance